Western blot vs. Elisa: which of these two tests is the most accurate for HIV testing?

Western blot vs. Elisa: which of these two tests is the most accurate for HIV testing?

Post Views: 801 Today, advanced medical research and innovative molecular diagnostic technologies can efficiently control, treat and even cure many deadly diseases. Among them is the Human Immunodeficiency Virus, commonly known as HIV, which, as the name suggests, attacks our immune system. Often confused with AIDS (we debunk this myth in one of our previous…

ELISA vs western blot: a comparison of two common immunoassays

ELISA vs western blot: a comparison of two common immunoassays

Post Views: 1,678 In biomedical research, the word “immunoassay” is common terminology. That there are different types of immunoassays is common knowledge for someone working in a laboratory. What is an immunoassay?     Immunoassay is a technique that evaluates the presence and concentration of a macromolecule in a given solution with the help of…

Aiming at Cytokines with ELISAs

Aiming at Cytokines with ELISAs

Post Views: 1,475 The study of cytokines is valuable in investigating inflammation. Cytokines are small proteins that transmit signals from cell to cell and trigger immunity to combat infection, inflammation and other disorders. Accurate detection and quantification of cytokine concentrations, therefore, becomes essential in describing the nature of the infection and powerful to follow the…

Which Controls to Use in ELISA Assays?

Which Controls to Use in ELISA Assays?

Post Views: 6,929 What is an ELISA? Enzyme-linked immunosorbent assay is popularly abbreviated as ELISA and has another name, enzyme immunoassay (EIA), defining the same technology. It’s a plate-based assay technique developed to discover and quantify soluble substances such as proteins, antibodies, peptides and hormones.  In an ELISA, the target macromolecule (the antigen) is palsied…

Enzyme-linked Immunosorbent Assay Principle and Elisa Test Procedures.

Enzyme-linked Immunosorbent Assay Principle and Elisa Test Procedures.

Post Views: 9,680 Enzyme-linked immunosorbent assay (ELISA) is also known as an enzyme immunoassay (EIA). ELISA is defined as a biochemical technique used in many applications, including microbiology, blood screening, veterinary, and immunology, to detect antigens and antibodies present in a sample. ELISA technology is considered an essential diagnostic tool in plant and medicine pathology.…