Mouse Heparan Sulfate ELISA Kit

Mouse Heparan Sulfate ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse Heparan Sulfate in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Competitive ELISA, Reactivity:Mouse, Product range:31.25-2000 ng/ml, Sensitivity:18.75 ng/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:Heparan Sulfate, Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Mouse Heparan Sulfate ELISA Kit (A73809) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse Heparan Sulfate in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Heparan Sulfate antigen. During the incubation, Heparan Sulfate present in the samples or standards competes with the fixed amount of immobilized Heparan Sulfate for binding sites on the Biotinylated Anti-Heparan Sulfate Antibody. The more Heparan Sulfate present in a sample or standard, the less Biotinylated Anti-Heparan Sulfate Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Heparan Sulfate Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Heparan Sulfate present in each sample or standard. The concentration of Heparan Sulfate can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Competitive (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.