Sheep Prolactin / PRL ELISA Kit

Sheep Prolactin / PRL ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of sheep Prolactin / PRL in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Competitive ELISA, Reactivity:Sheep, Product range:0.313-20 ng/ml, Sensitivity:0.188 ng/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:Prolactin / PRL, Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Sheep Prolactin / PRL ELISA Kit (A87526) employs the competitive enzyme immunoassay technique for the quantitative measurement of sheep Prolactin / PRL in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Prolactin / PRL antigen. During the incubation, Prolactin / PRL present in the samples or standards competes with the fixed amount of immobilized Prolactin / PRL for binding sites on the Biotinylated Anti-Prolactin / PRL Antibody. The more Prolactin / PRL present in a sample or standard, the less Biotinylated Anti-Prolactin / PRL Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Prolactin / PRL Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Prolactin / PRL present in each sample or standard. The concentration of Prolactin / PRL can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Competitive (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.