Human Sarcomeric alpha Actinin ELISA Kit

Human Sarcomeric alpha Actinin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Sarcomeric alpha Actinin in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Sandwich ELISA, Host:, Reactivity:Human, Product range:0.313-20 ng/ml, Sensitivity:0.188 ng/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:Sarcomeric alpha Actinin, Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Human Sarcomeric alpha Actinin ELISA Kit (A75158) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Sarcomeric alpha Actinin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Sarcomeric alpha Actinin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Sarcomeric alpha Actinin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Sarcomeric alpha Actinin Antibody, which binds the captured Sarcomeric alpha Actinin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Sarcomeric alpha Actinin captured in each well. The concentration of Sarcomeric alpha Actinin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Sandwich (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.