Mouse HMGB1 ELISA Kit

Mouse HMGB1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse HMGB1 in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Sandwich ELISA, Host:, Reactivity:Mouse, Product range:15.625-1000 pg/ml, Sensitivity:9.375 pg/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:HMGB1, Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Mouse HMGB1 ELISA Kit (A76689) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse HMGB1 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for HMGB1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HMGB1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HMGB1 Antibody, which binds the captured HMGB1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HMGB1 captured in each well. The concentration of HMGB1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Sandwich (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.