dsGreen for Real-Time PCR

dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allows the use of dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient. Unlike other preparations of dsGreen for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:. Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot. PCR tested preparation – quality guaranteed. Low fluorescence background – high fluorescence intensity gain.

Concentration:100X, Product form:Light orange solution, Purity:95% (by ¹H NMR, HPLC-MS, and PCR testing), Storage:Shipped at room temperature. Upon delivery, store in the dark at -20?C. Avoid prolonged exposure to light, Absorption maxima:454 nm, Extinction coefficient:73000 M^-1cm^-1, Emission maxima:524 nm, Fl quantum yield:0,8

+25° C.