Human Agrin (C-terminal fragment) ELISA Kit

Human Agrin (C-terminal fragment) ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Agrin (C-terminal fragment) in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Sandwich ELISA, Reactivity:Human, Product range:46.875-3000 pg/ml, Sensitivity:28.125 pg/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:Agrin (C-terminal fragment), Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Human Agrin (C-terminal fragment) ELISA Kit (A87452) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Agrin (C-terminal fragment) in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Agrin (C-terminal fragment) has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Agrin (C-terminal fragment) present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Agrin (C-terminal fragment) Antibody, which binds the captured Agrin (C-terminal fragment) present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Agrin (C-terminal fragment) captured in each well. The concentration of Agrin (C-terminal fragment) can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Sandwich (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.