Unlock special prices! Log in or register to reveal exclusive offers.
hhclogoproducts-1-jpg

Human PF4 ELISA Kit

The prices will be displayed on the checkout.

Category: Tag:

Human PF4 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human PF4 in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Sandwich ELISA, Reactivity:Human, Product range:1.563-100 ng/ml, Sensitivity:0.938 ng/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:PF4, Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Human PF4 ELISA Kit (A79251) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human PF4 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for PF4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PF4 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PF4 Antibody, which binds the captured PF4 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PF4 captured in each well. The concentration of PF4 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Sandwich (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.

FacebookTwitterLinkedin

Additional Information

Product Code

Origin

USA

BTN - HC

Size

KGS

Shipping Conditions

0
    Your Cart
    Your cart is emptyReturn to Shop

    Unlock special prices!
    Log in or register to reveal exclusive offers.