Unlock special prices! Log in or register to reveal exclusive offers.
hhclogoproducts-1-jpg

Human TNF alpha ELISA Kit

The prices will be displayed on the checkout.

Category: Tag:

Applications:ELISA, Reactivity:Human, Product range:3.16-1000 pg/ml, Sensitivity:2 pg/ml, Storage:Shipped at 4?C. Store the standard at -20?C; avoid freeze / thaw cycles. Store all other reagents at 2-8?C, Target:TNF alpha, Sample type:Serum, plasma, and cell culture supernatant, Principle assay:This Human TNF alpha ELISA Kit applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TNF alpha. Standards or samples are then added to the appropriate microtiter plate wells and incubated. TNF alpha if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound TNF alpha and other components of the sample. A biotinylated Anti-TNF alpha detection antibody is then added followed by Streptavidin-HRP. Addition of TMB substrate will result in a colored substrate product. The reaction is stopped with sulfuric acid and the optical density can be quantified using an ELISA plate reader. The concentration of analyte is determined by comparison to a serial dilution of the ELISA standard in parallel, Assay type:Sandwich (quantitative), Detection type:Colorimetric, Platform:Microplate

+25° C.

FacebookTwitterLinkedin

Additional Information

Product Code

Origin

USA

BTN - HC

Size

KGS

Shipping Conditions

0
    Your Cart
    Your cart is emptyReturn to Shop

    Unlock special prices!
    Log in or register to reveal exclusive offers.