Human Villin ELISA Kit

Human Villin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Villin in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Sandwich ELISA, Reactivity:Human, Product range:0.156-10 ng/ml, Sensitivity:0.094 ng/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:Villin, Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Human Villin ELISA Kit (A77494) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Villin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Villin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Villin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Villin Antibody, which binds the captured Villin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Villin captured in each well. The concentration of Villin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Sandwich (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.