Platelet Activating Factor ELISA Kit

Platelet Activating Factor ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Platelet Activating Factor in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Competitive ELISA, Reactivity:Universal, Product range:0.156-10 ng/ml, Sensitivity:0.094 ng/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:Platelet Activating Factor, Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Platelet Activating Factor ELISA Kit (A87286) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Platelet Activating Factor in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Platelet Activating Factor antigen. During the incubation, Platelet Activating Factor present in the samples or standards competes with the fixed amount of immobilized Platelet Activating Factor for binding sites on the Biotinylated Anti-Platelet Activating Factor Antibody. The more Platelet Activating Factor present in a sample or standard, the less Biotinylated Anti-Platelet Activating Factor Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Platelet Activating Factor Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Platelet Activating Factor present in each sample or standard. The concentration of Platelet Activating Factor can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Competitive (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.