Rat Serum Amyloid A ELISA Kit

Rat Serum Amyloid A ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Serum Amyloid A in serum, plasma, tissue homogenates, and other biological fluids.

Applications:Sandwich ELISA, Host:, Reactivity:Rat, Product range:15.625-1000 pg/ml, Sensitivity:9.375 pg/ml, Storage:Store at +4?C. Do not use past expiration date!, Target:Serum Amyloid A, Sample type:Serum, plasma, tissue homogenates, and other biological fluids, Principle assay:Rat Serum Amyloid A ELISA Kit (A80154) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Serum Amyloid A in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Serum Amyloid A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Serum Amyloid A present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Serum Amyloid A Antibody, which binds the captured Serum Amyloid A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Serum Amyloid A captured in each well. The concentration of Serum Amyloid A can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve, Assay type:Sandwich (quantitative), Detection type:Colorimetric, Platform:Pre-coated Microplate (12 x 8 well strips), Assay time:4h 30m

+25° C.